Species:

Rat

Alternative Name:

Pyrilinks-D, deoxyPYD

Uniport ID:

O89000

Assay Type:

Competitive

unit:

ng/mL

Standard:

200ng/mL

Sensitivity:

1.88 ng/mL

Detection Range:

3.13-200ng/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Research:

Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science;

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat DPD. During the reaction, Rat DPD in the sample or standard competes with a fixed amount of Rat DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat DPD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat DPD in tested samples can be calculated by comparing the OD of the samples to the standard curve.